Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Analysis of sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors.

Developmental biology | Feb 15, 2012

http://www.ncbi.nlm.nih.gov/pubmed/22185793

Development of the head skeleton involves reciprocal interactions between cranial neural crest cells (CNCCs) and the surrounding pharyngeal endoderm and ectoderm. Whereas elegant experiments in avians have shown a prominent role for the endoderm in facial skeleton development, the relative functions of the endoderm in growth versus regional identity of skeletal precursors have remained unclear. Here we describe novel craniofacial defects in zebrafish harboring mutations in the Sphingosine-1-phospate (S1P) type 2 receptor (s1pr2) or the S1P transporter Spinster 2 (spns2), and we show that S1P signaling functions in the endoderm for the proper growth and positioning of the jaw skeleton. Surprisingly, analysis of s1pr2 and spns2 mutants, as well as sox32 mutants that completely lack endoderm, reveals that the dorsal-ventral (DV) patterning of jaw skeletal precursors is largely unaffected even in the absence of endoderm. Instead, we observe reductions in the ectodermal expression of Fibroblast growth factor 8a (Fgf8a), and transgenic misexpression of Shha restores fgf8a expression and partially rescues the growth and differentiation of jaw skeletal precursors. Hence, we propose that the S1P-dependent anterior foregut endoderm functions primarily through Shh to regulate the growth but not DV patterning of zebrafish jaw precursors.

Pubmed ID: 22185793 RIS Download

Mesh terms: Analysis of Variance | Animals | Body Patterning | Carrier Proteins | Craniofacial Abnormalities | DNA Primers | DNA, Complementary | Endoderm | Fibroblast Growth Factors | Genotype | Hedgehog Proteins | Image Interpretation, Computer-Assisted | In Situ Hybridization | Jaw | Lysophospholipids | Membrane Proteins | Microscopy, Fluorescence | Mutation | Receptors, Lysosphingolipid | Signal Transduction | Sphingosine | Zebrafish | Zebrafish Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIDCR NIH HHS, Id: DE018405
  • Agency: NIDCR NIH HHS, Id: DE018405-03S1
  • Agency: NIDCR NIH HHS, Id: DE13834
  • Agency: NICHD NIH HHS, Id: HD22486
  • Agency: NHLBI NIH HHS, Id: HL54737
  • Agency: NICHD NIH HHS, Id: P01 HD022486
  • Agency: NIDCR NIH HHS, Id: R01 DE018405
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-01
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-02
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-03
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-03S1
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-04
  • Agency: NIDCR NIH HHS, Id: R01 DE018405-05

ZFIN (Data, Gene Expression)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.