Replication-coupled chromatin assembly generates a neuronal bilateral asymmetry in C. elegans.
Although replication-coupled chromatin assembly is known to be important for the maintenance of patterns of gene expression through sequential cell divisions, the role of replication-coupled chromatin assembly in controlling cell differentiation during animal development remains largely unexplored. Here we report that the CAF-1 protein complex, an evolutionarily conserved histone chaperone that deposits histone H3-H4 proteins onto replicating DNA, is required to generate a bilateral asymmetry in the C. elegans nervous system. A mutation in 1 of 24 C. elegans histone H3 genes specifically eliminates this aspect of neuronal asymmetry by causing a defect in the formation of a histone H3-H4 tetramer and the consequent inhibition of CAF-1-mediated nucleosome formation. Our results reveal that replication-coupled nucleosome assembly is necessary to generate a bilateral asymmetry in C. elegans neuroanatomy and suggest that left-right asymmetric epigenetic regulation can establish bilateral asymmetry in the nervous system.
Pubmed ID: 22177093 RIS Download
Amino Acid Sequence | Animals | Body Patterning | Caenorhabditis elegans | Caenorhabditis elegans Proteins | Chromatin Assembly and Disassembly | DNA Replication | Epigenomics | Histones | Molecular Sequence Data | Nervous System | Neurons | Nucleosomes | Sequence Alignment