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Protein kinase C η is required for T cell activation and homeostatic proliferation.

Science signaling | Dec 14, 2011

http://www.ncbi.nlm.nih.gov/pubmed/22155788

Protein kinase C η (PKCη) is abundant in T cells and is recruited to the immunological synapse that is formed between a T cell and an antigen-presenting cell; however, its function in T cells is unknown. We showed that PKCη was required for the activation of mature CD8+ T cells through the T cell receptor. Compared with wild-type T cells, PKCη-/- T cells showed poor proliferation in response to antigen stimulation, a trait shared with T cells deficient in PKCθ, which is the most abundant PKC isoform in T cells and was thought to be the only PKC isoform with a specific role in T cell activation. In contrast, only PKCη-deficient T cells showed defective homeostatic proliferation, which requires self-antigen recognition. PKCη was dispensable for thymocyte development; however, thymocytes from mice doubly deficient in PKCη and PKCθ exhibited poor development, indicating some redundancy between the PKC isoforms. Deficiency in PKCη or PKCθ had opposing effects on the relative numbers of CD4+ and CD8+ T cells. PKCη-/- mice had a higher ratio of CD4+ to CD8+ T cells compared to that of wild-type mice, whereas PKCθ-/- mice had a lower ratio. Mice deficient in both isoforms exhibited normal cell ratios. Together, these data suggest that PKCη shares some redundant roles with PKCθ in T cell biology and also performs nonredundant functions that are required for T cell homeostasis and activation.

Pubmed ID: 22155788 RIS Download

Mesh terms: Animals | Base Sequence | CD4-CD8 Ratio | CD4-Positive T-Lymphocytes | CD8-Positive T-Lymphocytes | Calcium Signaling | Cell Proliferation | Homeostasis | Immunologic Memory | Immunological Synapses | Isoenzymes | Lymphocyte Activation | Mice | Mice, Inbred C57BL | Mice, Knockout | NF-kappa B | Phenotype | Protein Kinase C | RNA, Messenger | Signal Transduction | T-Lymphocytes

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Associated grants

  • Agency: NIAID NIH HHS, Id: AI070845
  • Agency: NIGMS NIH HHS, Id: GM048002
  • Agency: NIGMS NIH HHS, Id: GM065230
  • Agency: NIH HHS, Id: OD006433
  • Agency: NIAID NIH HHS, Id: R01 AI070845
  • Agency: NIGMS NIH HHS, Id: R01 GM048002
  • Agency: NIGMS NIH HHS, Id: R01 GM048002-16
  • Agency: NIGMS NIH HHS, Id: R01 GM065230
  • Agency: NIGMS NIH HHS, Id: R01 GM065230-08
  • Agency: NIGMS NIH HHS, Id: R01 GM065230-09A2
  • Agency: NIGMS NIH HHS, Id: R01 GM088647
  • Agency: NIGMS NIH HHS, Id: R01 GM100785
  • Agency: NIAID NIH HHS, Id: T32AI07244
  • Agency: NHLBI NIH HHS, Id: T32HL07195

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