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The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.

PLoS genetics | Dec 6, 2011

http://www.ncbi.nlm.nih.gov/pubmed/22144917

Coordinated replication of eukaryotic genomes is intrinsically asymmetric, with continuous leading strand synthesis preceding discontinuous lagging strand synthesis. Here we provide two types of evidence indicating that, in fission yeast, these two biosynthetic tasks are performed by two different replicases. First, in Schizosaccharomyces pombe strains encoding a polδ-L591M mutator allele, base substitutions in reporter genes placed in opposite orientations relative to a well-characterized replication origin are strand-specific and distributed in patterns implying that Polδ is primarily involved in lagging strand replication. Second, in strains encoding a polε-M630F allele and lacking the ability to repair rNMPs in DNA due to a defect in RNase H2, rNMPs are selectively observed in nascent leading strand DNA. The latter observation demonstrates that abundant rNMP incorporation during replication can be tolerated and that they are normally removed in an RNase H2-dependent manner. This provides strong physical evidence that Polε is the primary leading strand replicase. Collectively, these data and earlier results in budding yeast indicate that the major roles of Polδ and Polε at the eukaryotic replication fork are evolutionarily conserved.

Pubmed ID: 22144917 RIS Download

Mesh terms: Base Sequence | Cdc20 Proteins | Cell Cycle Proteins | DNA Replication | Evolution, Molecular | Genome, Fungal | Molecular Sequence Data | Orotic Acid | Point Mutation | Replication Origin | Ribonuclease H | Schizosaccharomyces | Schizosaccharomyces pombe Proteins

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Associated grants

  • Agency: Medical Research Council, Id: G0801078
  • Agency: NIEHS NIH HHS, Id: Z01 ES065070
  • Agency: Intramural NIH HHS, Id:

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