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Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish.

Analysis of zebrafish mutants that have defects in motor behavior can allow entrée into the hindbrain and spinal cord networks that control locomotion. Here, we report that zebrafish techno trousers (tnt) locomotor mutants harbor a mutation in slc1a2b, which encodes Eaat2b, a plasma membrane glutamate transporter. We used tnt mutants to explore the effects of impaired glutamate transporter activity on locomotor network function. Wild-type larvae perform robust swimming behavior in response to touch stimuli at two and four days after fertilization. In contrast, tnt mutant larvae demonstrate aberrant, exaggerated body bends beginning two days after fertilization and they are almost paralyzed four days after fertilization. We show that slc1a2b is expressed in glial cells in a dynamic fashion across development, which may explain the abnormal sequence of motor behaviors demonstrated by tnt mutants. We also show that tnt larvae demonstrate enhanced excitation of neurons, consistent with the predicted effects of excessive glutamate. These findings illustrate the dynamic regulation and importance of glutamate transporters during development. Since glutamate toxicity caused by EAAT2 dysfunction is thought to promote several different neurological disorders in humans, including epilepsy and neurodegenerative diseases, tnt mutants hold promise as a new tool to better understand these pathologies.

Pubmed ID: 22094018


  • McKeown KA
  • Moreno R
  • Hall VL
  • Ribera AB
  • Downes GB


Developmental biology

Publication Data

February 15, 2012

Associated Grants

  • Agency: NINDS NIH HHS, Id: F32NS-059120
  • Agency: NINDS NIH HHS, Id: K01NS-057409
  • Agency: NINDS NIH HHS, Id: P30NS-048154
  • Agency: NINDS NIH HHS, Id: R01 NS025217
  • Agency: NINDS NIH HHS, Id: R01NS-025217
  • Agency: NINDS NIH HHS, Id: R01NS-038937
  • Agency: NCRR NIH HHS, Id: R01RR-020357

Mesh Terms

  • Animals
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary
  • Dyskinesias
  • Electrophysiology
  • Excitatory Amino Acid Transporter 2
  • Gene Expression Regulation, Developmental
  • Glutamic Acid
  • In Situ Hybridization
  • Larva
  • Morpholinos
  • Mutation
  • Neuroglia
  • Neurons
  • Physical Stimulation
  • Swimming
  • Video Recording
  • Zebrafish