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The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.

http://www.ncbi.nlm.nih.gov/pubmed/22081013

RNA interference (RNAi) is critical for the assembly of heterochromatin at Schizosaccharomyces pombe centromeres. Central to this process is the RNA-induced initiation of transcriptional gene silencing (RITS) complex, which physically anchors small noncoding RNAs to chromatin. RITS includes Ago1, the chromodomain protein Chp1, and Tas3, which forms a bridge between Chp1 and Ago1. Chp1 is a large protein with no recognizable domains, apart from its chromodomain. Here we describe how the structured C-terminal half of Chp1 binds the Tas3 N-terminal domain, revealing the tight association of Chp1 and Tas3. The structure also shows a PIN domain at the C-terminal tip of Chp1 that controls subtelomeric transcripts through a post-transcriptional mechanism. We suggest that the Chp1-Tas3 complex provides a solid and versatile platform to recruit both RNAi-dependent and RNAi-independent gene-silencing pathways for locus-specific regulation of heterochromatin.

Pubmed ID: 22081013 RIS Download

Mesh terms: Amino Acid Sequence | Argonaute Proteins | Carrier Proteins | Cell Cycle Proteins | Gene Silencing | Models, Molecular | Multigene Family | Protein Interaction Mapping | Protein Structure, Tertiary | RNA Interference | Schizosaccharomyces | Schizosaccharomyces pombe Proteins | Sequence Alignment | Sequence Analysis, Protein

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Associated grants

  • Agency: NCI NIH HHS, Id: P30 CA045508
  • Agency: NCI NIH HHS, Id: P30CA021765
  • Agency: NIGMS NIH HHS, Id: R01 GM084045
  • Agency: NIGMS NIH HHS, Id: R01 GM084045-04
  • Agency: NIGMS NIH HHS, Id: R01GM084045

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