Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Signal-dependent incorporation of MyoD-BAF60c into Brg1-based SWI/SNF chromatin-remodelling complex.

The EMBO journal | Jan 18, 2012

Tissue-specific transcriptional activators initiate differentiation towards specialized cell types by inducing chromatin modifications permissive for transcription at target loci, through the recruitment of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodelling complex. However, the molecular mechanism that regulates SWI/SNF nuclear distribution in response to differentiation signals is unknown. We show that the muscle determination factor MyoD and the SWI/SNF subunit BAF60c interact on the regulatory elements of MyoD-target genes in myoblasts, prior to activation of transcription. BAF60c facilitates MyoD binding to target genes and marks the chromatin for signal-dependent recruitment of the SWI/SNF core to muscle genes. BAF60c phosphorylation on a conserved threonine by differentiation-activated p38α kinase is the signal that promotes incorporation of MyoD-BAF60c into a Brg1-based SWI/SNF complex, which remodels the chromatin and activates transcription of MyoD-target genes. Our data support an unprecedented two-step model by which pre-assembled BAF60c-MyoD complex directs recruitment of SWI/SNF to muscle loci in response to differentiation cues.

Pubmed ID: 22068056 RIS Download

Mesh terms: Animals | Cell Line | Chromatin | Chromosomal Proteins, Non-Histone | DNA Helicases | Fibroblasts | Gene Expression Regulation | HeLa Cells | Humans | MAP Kinase Signaling System | Mice | Multiprotein Complexes | Muscle Development | Muscle Proteins | MyoD Protein | Myoblasts | Nuclear Proteins | Phosphorylation | Phosphothreonine | Protein Processing, Post-Translational | RNA Interference | RNA, Small Interfering | Transcription Factors | Two-Hybrid System Techniques | p38 Mitogen-Activated Protein Kinases

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAMS NIH HHS, Id: R01 AR052779-06A1
  • Agency: Telethon, Id: TCR05004
  • Agency: NIAMS NIH HHS, Id: R01 AR056712-03
  • Agency: NIAMS NIH HHS, Id: AR053779
  • Agency: NIAMS NIH HHS, Id: R01 AR056712
  • Agency: NIAMS NIH HHS, Id: R01 AR052779

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.