Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Sub1 and RPA associate with RNA polymerase II at different stages of transcription.

Molecular cell | Nov 4, 2011

http://www.ncbi.nlm.nih.gov/pubmed/22055186

Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. Quantitative proteomic analysis of RNA polymerase II (RNApII) preinitiation complexes (PICs) identified Sub1 and the replication protein A complex (RPA), both of which bind single-stranded DNA (ssDNA). Sub1, homolog of mammalian coactivator PC4, exhibits strong genetic interactions with factors necessary for promoter melting. Sub1 localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon PIC assembly. In contrast, RPA localizes to transcribed regions of active genes, strongly correlated with transcribing RNApII but independently of replication. RFA1 interacts genetically with transcription elongation factor genes. Interestingly, RPA levels increase at active promoters in cells carrying a Sub1 deletion or ssDNA-binding mutant, suggesting competition for a common binding site. We propose that Sub1 and RPA interact with the nontemplate strand of RNApII complexes during initiation and elongation, respectively.

Pubmed ID: 22055186 RIS Download

Mesh terms: Base Sequence | Binding Sites | DNA, Single-Stranded | DNA-Binding Proteins | Gene Expression Regulation, Fungal | Molecular Sequence Data | Peptide Chain Elongation, Translational | Peptide Chain Initiation, Translational | Promoter Regions, Genetic | Proteomics | RNA Polymerase II | Replication Protein A | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription Factors | Transcription Initiation Site | Transcription, Genetic

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM079205
  • Agency: NIGMS NIH HHS, Id: GM46498
  • Agency: NIGMS NIH HHS, Id: R01 GM046498
  • Agency: NIGMS NIH HHS, Id: R01 GM046498-19S1
  • Agency: NIGMS NIH HHS, Id: R01 GM046498-20
  • Agency: NIGMS NIH HHS, Id: R01 GM046498-21
  • Agency: NIGMS NIH HHS, Id: R01 GM056663
  • Agency: NIGMS NIH HHS, Id: R01 GM079205

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.