Detailed analysis of the synaptic inputs to the primate DB1 bipolar cell has been precluded by the absence of a suitable immunohistochemical marker. Here we demonstrate that antibodies for the EF-hand calcium-binding protein, secretagogin, strongly label the DB1 bipolar cell as well as a mixed population of GABAergic amacrine cells in the macaque retina. Using secretagogin as a marker, we show that the DB1 bipolar makes synaptic contact with both L/M as well as S-cone photoreceptors and only minimal contact with rod photoreceptors. Electron microscopy showed that the DB1 bipolar makes flat contacts at both triad-associated and nontriad-associated positions on the cone pedicle. Double labeling with various glutamate receptor subunit antibodies failed to conclusively determine the subunit composition of the glutamate receptors on DB1 bipolar cells. In the IPL, DB1 bipolar cell axon terminals expressed the glycine receptor, GlyRα1, at sites of contact with AII amacrine cells, suggesting that these cells receive input from the rod pathway.
Pubmed ID: 22006647 RIS Download
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This polyclonal targets TYROSINE HYDROXYLASE
View all literature mentionsThis polyclonal targets Human Secretagogin
View all literature mentionsThis polyclonal targets Human Secretagogin
View all literature mentionsThis polyclonal targets VGLUT1
View all literature mentionsThis unknown targets
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View all literature mentionsThis monoclonal targets Synthetic peptide containing 15 of the last 16 amino acids at the extreme C-terminus of the human beta-dystroglycan sequence (PKNMTPYRSPPPYVP-PCOOH).
View all literature mentionsThis monoclonal targets Calbindin-D-28K antibody produced in mouse
View all literature mentionsThis polyclonal targets Human GRIK1
View all literature mentionsThis monoclonal targets Glycine receptor alpha 1
View all literature mentionsThis polyclonal targets GABA
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