• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population.

The neural crest is a stem cell-like population exclusive to vertebrates that gives rise to many different cell types including chondrocytes, neurons and melanocytes. Arising from the neural plate border at the intersection of Wnt and Bmp signaling pathways, the complexity of neural crest gene regulatory networks has made the earliest steps of induction difficult to elucidate. Here, we report that tfap2a and foxd3 participate in neural crest induction and are necessary and sufficient for this process to proceed. Double mutant tfap2a (mont blanc, mob) and foxd3 (mother superior, mos) mob;mos zebrafish embryos completely lack all neural crest-derived tissues. Moreover, tfap2a and foxd3 are expressed during gastrulation prior to neural crest induction in distinct, complementary, domains; tfap2a is expressed in the ventral non-neural ectoderm and foxd3 in the dorsal mesendoderm and ectoderm. We further show that Bmp signaling is expanded in mob;mos embryos while expression of dkk1, a Wnt signaling inhibitor, is increased and canonical Wnt targets are suppressed. These changes in Bmp and Wnt signaling result in specific perturbations of neural crest induction rather than general defects in neural plate border or dorso-ventral patterning. foxd3 overexpression, on the other hand, enhances the ability of tfap2a to ectopically induce neural crest around the neural plate, overriding the normal neural plate border limit of the early neural crest territory. Although loss of either Tfap2a or Foxd3 alters Bmp and Wnt signaling patterns, only their combined inactivation sufficiently alters these signaling gradients to abort neural crest induction. Collectively, our results indicate that tfap2a and foxd3, in addition to their respective roles in the differentiation of neural crest derivatives, also jointly maintain the balance of Bmp and Wnt signaling in order to delineate the neural crest induction domain.

Pubmed ID: 21963426


  • Wang WD
  • Melville DB
  • Montero-Balaguer M
  • Hatzopoulos AK
  • Knapik EW


Developmental biology

Publication Data

December 1, 2011

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL083958
  • Agency: NHLBI NIH HHS, Id: HL100398
  • Agency: NIDCR NIH HHS, Id: R01 DE018477
  • Agency: NIDCR NIH HHS, Id: R01 DE018477-01
  • Agency: NIDCR NIH HHS, Id: R01 DE018477-02
  • Agency: NIDCR NIH HHS, Id: R01 DE018477-03
  • Agency: NIDCR NIH HHS, Id: R01 DE018477-04
  • Agency: NHLBI NIH HHS, Id: R01 HL083958
  • Agency: NHLBI NIH HHS, Id: R01 HL083958-03
  • Agency: NHLBI NIH HHS, Id: R01 HL083958-04
  • Agency: NHLBI NIH HHS, Id: R01 HL083958-04S1
  • Agency: NHLBI NIH HHS, Id: R01 HL083958-05
  • Agency: NHLBI NIH HHS, Id: U01 HL100398
  • Agency: NHLBI NIH HHS, Id: U01 HL100398-01
  • Agency: NHLBI NIH HHS, Id: U01 HL100398-02

Mesh Terms

  • Animals
  • Base Sequence
  • Body Patterning
  • Bone Morphogenetic Proteins
  • Cell Death
  • DNA Primers
  • Embryonic Stem Cells
  • Forkhead Transcription Factors
  • Gastrulation
  • Gene Expression Regulation, Developmental
  • Genes, p53
  • Intercellular Signaling Peptides and Proteins
  • Mutation
  • Neural Crest
  • Neurogenesis
  • Transcription Factor AP-2
  • Wnt Signaling Pathway
  • Zebrafish
  • Zebrafish Proteins