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Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain.

PLoS genetics | Sep 13, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21912526

The Tsx gene resides at the X-inactivation center and is thought to encode a protein expressed in testis, but its function has remained mysterious. Given its proximity to noncoding genes that regulate X-inactivation, here we characterize Tsx and determine its function in mice. We find that Tsx is actually noncoding and the long transcript is expressed robustly in meiotic germ cells, embryonic stem cells, and brain. Targeted deletion of Tsx generates viable offspring and X-inactivation is only mildly affected in embryonic stem cells. However, mutant embryonic stem cells are severely growth-retarded, differentiate poorly, and show elevated cell death. Furthermore, male mice have smaller testes resulting from pachytene-specific apoptosis and a maternal-specific effect results in slightly smaller litters. Intriguingly, male mice lacking Tsx are less fearful and have measurably enhanced hippocampal short-term memory. Combined, our study indicates that Tsx performs general functions in multiple cell types and links the noncoding locus to stem and germ cell development, learning, and behavior in mammals.

Pubmed ID: 21912526 RIS Download

Mesh terms: Animals | Apoptosis | Brain | Embryonic Stem Cells | Female | Gene Expression Regulation, Developmental | Germ Cells | Hippocampus | Male | Memory, Short-Term | Mice | Proteins | RNA, Untranslated | Sequence Deletion | Testis | X Chromosome Inactivation

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Associated grants

  • Agency: NINDS NIH HHS, Id: R01 NS075346
  • Agency: Howard Hughes Medical Institute, Id:

Mouse Genome Informatics (Data, Gene Annotation)

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