We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

MicroRNAs can generate thresholds in target gene expression.

Nature genetics | Aug 21, 2011

MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress gene expression in a sequence-dependent manner. We performed single-cell measurements using quantitative fluorescence microscopy and flow cytometry to monitor a target gene's protein expression in the presence and absence of regulation by miRNA. We find that although the average level of repression is modest, in agreement with previous population-based measurements, the repression among individual cells varies dramatically. In particular, we show that regulation by miRNAs establishes a threshold level of target mRNA below which protein production is highly repressed. Near this threshold, protein expression responds sensitively to target mRNA input, consistent with a mathematical model of molecular titration. These results show that miRNAs can act both as a switch and as a fine-tuner of gene expression.

Pubmed ID: 21857679 RIS Download

Mesh terms: Binding Sites | Flow Cytometry | Gene Expression | Gene Expression Regulation | Gene Targeting | Genes, Reporter | HeLa Cells | Humans | Luciferases | MicroRNAs | Microscopy, Fluorescence

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

Associated grants

  • Agency: NCI NIH HHS, Id: DP1 CA174420
  • Agency: NCI NIH HHS, Id: R01-CA133404
  • Agency: NIH HHS, Id: 1DP1OD003936
  • Agency: NCI NIH HHS, Id: U54 CA143874-02
  • Agency: NCI NIH HHS, Id: P01 CA042063
  • Agency: NCI NIH HHS, Id: P30 CA014051
  • Agency: NCI NIH HHS, Id: P30-CA14051
  • Agency: NIH HHS, Id: DP1 OD003936-03
  • Agency: NCI NIH HHS, Id: U54 CA143874
  • Agency: NCI NIH HHS, Id: R01 CA133404
  • Agency: NCI NIH HHS, Id: U54CA143874
  • Agency: Howard Hughes Medical Institute, Id: DP1 OD003936
  • Agency: NIH HHS, Id: P01-CA42063
  • Agency: NCI NIH HHS, Id: DP1 OD003936-02
  • Agency: NIH HHS, Id: R01-GM34277
  • Agency: NIGMS NIH HHS, Id:

Addgene (Reagent, Plasmid)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.