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Eliminating SF-1 (NR5A1) sumoylation in vivo results in ectopic hedgehog signaling and disruption of endocrine development.

Developmental cell | Aug 16, 2011

Sumoylation is generally considered a repressive mark for many transcription factors. However, the in vivo importance of sumoylation for any given substrate remains unclear and is questionable because the extent of sumoylation appears exceedingly low for most substrates. Here, we permanently eliminated SF-1/NR5A1 sumoylation in mice (Sf-1(K119R, K194R, or 2KR)) and found that Sf-1(2KR/2KR) mice failed to phenocopy a simple gain of SF-1 function or show elevated levels of well-established SF-1 target genes. Instead, mutant mice exhibited marked endocrine abnormalities and changes in cell fate that reflected an inappropriate activation of hedgehog signaling and other potential SUMO-sensitive targets. Furthermore, unsumoylatable SF-1 mutants activated Shh and exhibited preferential recruitment to Shh genomic elements in cells. We conclude that the sumoylation cycle greatly expands the functional capacity of transcription factors such as SF-1 and is leveraged during development to achieve cell-type-specific gene expression in multicellular organisms.

Pubmed ID: 21820362 RIS Download

Mesh terms: Adrenal Glands | Animals | Animals, Newborn | Antigens, CD | Antigens, CD31 | Antigens, Differentiation, B-Lymphocyte | Carrier Proteins | Cells, Cultured | Corticosterone | Electrophoretic Mobility Shift Assay | Embryo, Mammalian | Endocrine System | Female | Gene Expression Profiling | Gene Expression Regulation, Developmental | Hedgehog Proteins | Immunoprecipitation | Kruppel-Like Transcription Factors | Leydig Cells | Male | Membrane Proteins | Mice | Mice, Inbred BALB C | Mice, Transgenic | Models, Biological | Mutation | Oligonucleotide Array Sequence Analysis | SOX9 Transcription Factor | Signal Transduction | Spermatozoa | Steroidogenic Factor 1 | Sumoylation | Testis | Testosterone | Transfection | Zinc Finger Protein GLI1

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Associated grants

  • Agency: NIDDK NIH HHS, Id: T32 DK007418
  • Agency: NIDDK NIH HHS, Id: R01 DK070024-06
  • Agency: NIDDK NIH HHS, Id: R01 DK070024
  • Agency: NIDDK NIH HHS, Id: F32 DK079499-02
  • Agency: NIDDK NIH HHS, Id: T32 DK07418
  • Agency: Canadian Institutes of Health Research, Id: R01 DK070024-07
  • Agency: NIDDK NIH HHS, Id: F32 DK082100-03
  • Agency: NIDDK NIH HHS, Id:

Mouse Genome Informatics (Data, Gene Annotation)

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