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Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.

Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.

Pubmed ID: 21804561

Authors

  • Kelly OG
  • Chan MY
  • Martinson LA
  • Kadoya K
  • Ostertag TM
  • Ross KG
  • Richardson M
  • Carpenter MK
  • D'Amour KA
  • Kroon E
  • Moorman M
  • Baetge EE
  • Bang AG

Journal

Nature biotechnology

Publication Data

August 8, 2011

Associated Grants

None

Mesh Terms

  • Animals
  • Antibodies
  • Antigens, CD
  • Biological Markers
  • Cell Separation
  • Cells, Cultured
  • Embryonic Stem Cells
  • Endoderm
  • Flow Cytometry
  • Humans
  • Mice
  • Mice, SCID
  • Microscopy, Fluorescence
  • Pancreas
  • Transplantation, Heterologous