• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Synaptogenesis of electrical and GABAergic synapses of fast-spiking inhibitory neurons in the neocortex.

Parvalbumin-expressing fast-spiking (FS) cells are interconnected via GABAergic and electrical synapses and represent a major class of inhibitory interneurons in the neocortex. Synaptic connections among FS cells are critical for regulating network oscillations in the mature neocortex. However, it is unclear whether synaptic connections among FS interneurons also play a central role in the generation of patterned neuronal activity in the immature brain, which is thought to underlie the formation of neocortical circuits. Here, we investigated the developmental time course of synaptogenesis of FS cell in mouse visual cortex. In layer 5/6 (L5/6), we recorded from two or three FS and/or pyramidal (PYR) neurons to study the development of electrical and chemical synaptic interactions from postnatal day 3 (P3) to P18. We detected no evidence for functional connectivity for FS-FS or FS-PYR pairs at P3-P4. However, by P5-P6, we found that 20% of FS pairs were electrically coupled, and 24% of pairs were connected via GABAergic synapses; by P15-P18, 42% of FS pairs had established functional electrical synapses, and 47% of FS pairs were connected via GABAergic synapses. FS cell GABAergic inhibition of pyramidal cells showed a similar developmental time line, but no electrical coupling was detected for FS-PYR pairs. We found that synaptogenesis of electrical and GABAergic connections of FS cells takes place in the same period. Together, our results suggest that chemical and electrical connections among FS cells can contribute to patterned neocortical activity only by the end of the first postnatal week.

Pubmed ID: 21795529

Authors

  • Pangratz-Fuehrer S
  • Hestrin S

Journal

The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

July 27, 2011

Associated Grants

  • Agency: NEI NIH HHS, Id: R01 EY012114
  • Agency: NEI NIH HHS, Id: R01 EY012114-11A2

Mesh Terms

  • Action Potentials
  • Age Factors
  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Electrical Synapses
  • Glutamate Decarboxylase
  • Green Fluorescent Proteins
  • In Vitro Techniques
  • Inhibitory Postsynaptic Potentials
  • Mice
  • Mice, Transgenic
  • Neocortex
  • Neural Inhibition
  • Parvalbumins
  • Pyramidal Cells
  • Signal Transduction
  • gamma-Aminobutyric Acid