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Bright and stable near-infrared fluorescent protein for in vivo imaging.

Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency. Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes. Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra.

Pubmed ID: 21765402


  • Filonov GS
  • Piatkevich KD
  • Ting LM
  • Zhang J
  • Kim K
  • Verkhusha VV


Nature biotechnology

Publication Data

August 8, 2011

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI046985
  • Agency: NIAID NIH HHS, Id: AI087625
  • Agency: NIGMS NIH HHS, Id: GM073913
  • Agency: NIAID NIH HHS, Id: R01 AI087625
  • Agency: NIBIB NIH HHS, Id: R01 EB006432
  • Agency: NIGMS NIH HHS, Id: R01 GM073913
  • Agency: NIGMS NIH HHS, Id: R01 GM073913-06
  • Agency: NCRR NIH HHS, Id: S10 RR027308
  • Agency: NCRR NIH HHS, Id: S10RR027308

Mesh Terms

  • Adenoviridae
  • Animals
  • Diagnostic Imaging
  • Female
  • Flow Cytometry
  • Fluorescent Dyes
  • HeLa Cells
  • Humans
  • Luminescent Proteins
  • Mice
  • Mice, Inbred C57BL
  • Phantoms, Imaging
  • Phytochrome
  • Protein Stability
  • Spectrometry, Fluorescence