• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development.

The stability of mRNA influences the dynamics of gene expression. The mammalian CCR4-NOT complex is associated with deadenylase activity, which shortens the mRNA poly(A) tail and thereby contributes to destabilization of mRNAs. The complex consists of at least nine subunits and predominantly forms a 2.0MDa protein complex in HeLa cells. Accumulating evidence suggests that the CCR4-NOT complex is involved in cell growth and survival; however, the regulatory mechanisms of its biological activity remain obscure. Here, we analyzed the expression levels of the subunits of the CCR4-NOT complex in various mouse tissues and found that they showed distinct expression patterns. CNOT6, 6L, 7, and 10 were expressed nearly ubiquitously, whereas others were expressed in tissue-specific manners, such as those displaying especially high expression in the brain. Furthermore, CNOT2, 3, 6, and 8 were rapidly downregulated during differentiation of neural stem cells. These findings suggest that subunit composition of the CCR4-NOT complex differs among tissues and is altered during neural development, thereby imparting an additional layer of specificity in the control of gene expression.

Pubmed ID: 21741365

Authors

  • Chen C
  • Ito K
  • Takahashi A
  • Wang G
  • Suzuki T
  • Nakazawa T
  • Yamamoto T
  • Yokoyama K

Journal

Biochemical and biophysical research communications

Publication Data

July 29, 2011

Associated Grants

None

Mesh Terms

  • Animals
  • Cell Line
  • Mice
  • Mice, Mutant Strains
  • Multienzyme Complexes
  • Neural Stem Cells
  • Neurogenesis
  • Protein Subunits
  • RNA Stability
  • Rats
  • Rats, Wistar
  • Ribonucleases
  • Tissue Distribution
  • Transcription Factors