• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

Promoter-proximal pausing by initiated RNA polymerase II (Pol II) and regulated release of paused polymerase into productive elongation has emerged as a major mechanism of transcription activation. Reactivation of paused Pol II correlates with recruitment of super-elongation complexes (SECs) containing ELL/EAF family members, P-TEFb, and other proteins, but the mechanism of their recruitment is an unanswered question. Here, we present evidence for a role of human Mediator subunit MED26 in this process. We identify in the conserved N-terminal domain of MED26 overlapping docking sites for SEC and a second ELL/EAF-containing complex, as well as general initiation factor TFIID. In addition, we present evidence consistent with the model that MED26 can function as a molecular switch that interacts first with TFIID in the Pol II initiation complex and then exchanges TFIID for complexes containing ELL/EAF and P-TEFb to facilitate transition of Pol II into the elongation stage of transcription.

Pubmed ID: 21729782

Authors

  • Takahashi H
  • Parmely TJ
  • Sato S
  • Tomomori-Sato C
  • Banks CA
  • Kong SE
  • Szutorisz H
  • Swanson SK
  • Martin-Brown S
  • Washburn MP
  • Florens L
  • Seidel CW
  • Lin C
  • Smith ER
  • Shilatifard A
  • Conaway RC
  • Conaway JW

Journal

Cell

Publication Data

July 8, 2011

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM41628
  • Agency: NIGMS NIH HHS, Id: R01 GM041628
  • Agency: NIGMS NIH HHS, Id: R01 GM041628-23

Mesh Terms

  • Cell Proliferation
  • Gene Expression Regulation
  • HSP70 Heat-Shock Proteins
  • HeLa Cells
  • Humans
  • Mediator Complex
  • Phosphorylation
  • Proto-Oncogene Proteins c-myc
  • RNA Polymerase II
  • Trans-Activators
  • Transcription, Genetic
  • Transcriptional Elongation Factors