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Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

Cell | Jul 8, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21729782

Promoter-proximal pausing by initiated RNA polymerase II (Pol II) and regulated release of paused polymerase into productive elongation has emerged as a major mechanism of transcription activation. Reactivation of paused Pol II correlates with recruitment of super-elongation complexes (SECs) containing ELL/EAF family members, P-TEFb, and other proteins, but the mechanism of their recruitment is an unanswered question. Here, we present evidence for a role of human Mediator subunit MED26 in this process. We identify in the conserved N-terminal domain of MED26 overlapping docking sites for SEC and a second ELL/EAF-containing complex, as well as general initiation factor TFIID. In addition, we present evidence consistent with the model that MED26 can function as a molecular switch that interacts first with TFIID in the Pol II initiation complex and then exchanges TFIID for complexes containing ELL/EAF and P-TEFb to facilitate transition of Pol II into the elongation stage of transcription.

Pubmed ID: 21729782 RIS Download

Mesh terms: Cell Proliferation | Gene Expression Regulation | HSP70 Heat-Shock Proteins | HeLa Cells | Humans | Mediator Complex | Phosphorylation | Proto-Oncogene Proteins c-myc | RNA Polymerase II | Trans-Activators | Transcription, Genetic | Transcriptional Elongation Factors

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM41628
  • Agency: NIGMS NIH HHS, Id: R01 GM041628
  • Agency: NIGMS NIH HHS, Id: R01 GM041628-23

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