Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Probing individual environmental bacteria for viruses by using microfluidic digital PCR.

Science (New York, N.Y.) | Jul 1, 2011

Viruses may very well be the most abundant biological entities on the planet. Yet neither metagenomic studies nor classical phage isolation techniques have shed much light on the identity of the hosts of most viruses. We used a microfluidic digital polymerase chain reaction (PCR) approach to physically link single bacterial cells harvested from a natural environment with a viral marker gene. When we implemented this technique on the microbial community residing in the termite hindgut, we found genus-wide infection patterns displaying remarkable intragenus selectivity. Viral marker allelic diversity revealed restricted mixing of alleles between hosts, indicating limited lateral gene transfer of these alleles despite host proximity. Our approach does not require culturing hosts or viruses and provides a method for examining virus-bacterium interactions in many environments.

Pubmed ID: 21719670 RIS Download

Mesh terms: Alleles | Amino Acid Sequence | Animals | Bacteriophages | Ecosystem | Endodeoxyribonucleases | Genes, Viral | Genes, rRNA | Genetic Variation | Intestines | Isoptera | Microbial Interactions | Microfluidic Analytical Techniques | Molecular Sequence Data | Phylogeny | Polymerase Chain Reaction | Prophages | Sequence Alignment | Treponema

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.