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Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.

Science (New York, N.Y.) | Aug 5, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21719644

Tail-anchored (TA) proteins are involved in cellular processes including trafficking, degradation, and apoptosis. They contain a C-terminal membrane anchor and are posttranslationally delivered to the endoplasmic reticulum (ER) membrane by the Get3 adenosine triphosphatase interacting with the hetero-oligomeric Get1/2 receptor. We have determined crystal structures of Get3 in complex with the cytosolic domains of Get1 and Get2 in different functional states at 3.0, 3.2, and 4.6 angstrom resolution. The structural data, together with biochemical experiments, show that Get1 and Get2 use adjacent, partially overlapping binding sites and that both can bind simultaneously to Get3. Docking to the Get1/2 complex allows for conformational changes in Get3 that are required for TA protein insertion. These data suggest a molecular mechanism for nucleotide-regulated delivery of TA proteins.

Pubmed ID: 21719644 RIS Download

Mesh terms: Adaptor Proteins, Vesicular Transport | Adenosine Triphosphatases | Adenosine Triphosphate | Amino Acid Sequence | Binding Sites | Catalytic Domain | Crystallography, X-Ray | Cytosol | Endoplasmic Reticulum | Guanine Nucleotide Exchange Factors | Membrane Proteins | Microsomes | Models, Molecular | Molecular Sequence Data | Protein Binding | Protein Interaction Domains and Motifs | Protein Multimerization | Protein Structure, Secondary | Protein Structure, Tertiary | Protein Subunits | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins

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Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM099943

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