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A public genome-scale lentiviral expression library of human ORFs.

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Pubmed ID: 21706014


  • Yang X
  • Boehm JS
  • Yang X
  • Salehi-Ashtiani K
  • Hao T
  • Shen Y
  • Lubonja R
  • Thomas SR
  • Alkan O
  • Bhimdi T
  • Green TM
  • Johannessen CM
  • Silver SJ
  • Nguyen C
  • Murray RR
  • Hieronymus H
  • Balcha D
  • Fan C
  • Lin C
  • Ghamsari L
  • Vidal M
  • Hahn WC
  • Hill DE
  • Root DE


Nature methods

Publication Data

August 29, 2011

Associated Grants

  • Agency: NCI NIH HHS, Id: P01 CA142536
  • Agency: NCI NIH HHS, Id: R33 CA128625
  • Agency: NCI NIH HHS, Id: R33 CA128625
  • Agency: NCI NIH HHS, Id: R33 CA128625-01A1
  • Agency: NCI NIH HHS, Id: R33 CA128625-02
  • Agency: NCI NIH HHS, Id: R33 CA128625-03

Mesh Terms

  • Cloning, Molecular
  • Genetic Vectors
  • Genomic Library
  • Humans
  • Lentivirus
  • Open Reading Frames