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A public genome-scale lentiviral expression library of human ORFs.

Nature methods | Aug 29, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21706014

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Pubmed ID: 21706014 RIS Download

Mesh terms: Cloning, Molecular | Genetic Vectors | Genomic Library | Humans | Lentivirus | Open Reading Frames

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Associated grants

  • Agency: NCI NIH HHS, Id: P01 CA142536
  • Agency: NCI NIH HHS, Id: R33 CA128625
  • Agency: NCI NIH HHS, Id: R33 CA128625
  • Agency: NCI NIH HHS, Id: R33 CA128625-01A1
  • Agency: NCI NIH HHS, Id: R33 CA128625-02
  • Agency: NCI NIH HHS, Id: R33 CA128625-03

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