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Mutagenic processing of ribonucleotides in DNA by yeast topoisomerase I.

Science (New York, N.Y.) | Jun 24, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21700875

The ribonuclease (RNase) H class of enzymes degrades the RNA component of RNA:DNA hybrids and is important in nucleic acid metabolism. RNase H2 is specialized to remove single ribonucleotides [ribonucleoside monophosphates (rNMPs)] from duplex DNA, and its absence in budding yeast has been associated with the accumulation of deletions within short tandem repeats. Here, we demonstrate that rNMP-associated deletion formation requires the activity of Top1, a topoisomerase that relaxes supercoils by reversibly nicking duplex DNA. The reported studies extend the role of Top1 to include the processing of rNMPs in genomic DNA into irreversible single-strand breaks, an activity that can have distinct mutagenic consequences and may be relevant to human disease.

Pubmed ID: 21700875 RIS Download

Mesh terms: Amino Acid Transport Systems, Basic | Base Sequence | Camptothecin | Canavanine | DNA Breaks | DNA Topoisomerases, Type I | DNA, Fungal | DNA, Single-Stranded | Microsatellite Repeats | Molecular Sequence Data | Mutagenesis | Nucleic Acid Conformation | Ribonuclease H | Ribonucleotides | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Sequence Deletion | Transcription, Genetic

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Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM038464
  • Agency: NIGMS NIH HHS, Id: R01 GM093197
  • Agency: NIGMS NIH HHS, Id: R01 GM38464
  • Agency: NIGMS NIH HHS, Id: R01 GM93197
  • Agency: Intramural NIH HHS, Id:

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