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A cell cycle phosphoproteome of the yeast centrosome.

Science (New York, N.Y.) | Jun 24, 2011

Centrosomes organize the bipolar mitotic spindle, and centrosomal defects cause chromosome instability. Protein phosphorylation modulates centrosome function, and we provide a comprehensive map of phosphorylation on intact yeast centrosomes (18 proteins). Mass spectrometry was used to identify 297 phosphorylation sites on centrosomes from different cell cycle stages. We observed different modes of phosphoregulation via specific protein kinases, phosphorylation site clustering, and conserved phosphorylated residues. Mutating all eight cyclin-dependent kinase (Cdk)-directed sites within the core component, Spc42, resulted in lethality and reduced centrosomal assembly. Alternatively, mutation of one conserved Cdk site within γ-tubulin (Tub4-S360D) caused mitotic delay and aberrant anaphase spindle elongation. Our work establishes the extent and complexity of this prominent posttranslational modification in centrosome biology and provides specific examples of phosphorylation control in centrosome function.

Pubmed ID: 21700874 RIS Download

Mesh terms: Binding Sites | CDC2 Protein Kinase | Cell Cycle | Centrosome | Cytoskeletal Proteins | Fungal Proteins | Fungi | G1 Phase | Mitosis | Mutation | Phosphoproteins | Phosphorylation | Protein Processing, Post-Translational | Proteome | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Spindle Apparatus | Tubulin

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Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM051312-16S1
  • Agency: NIGMS NIH HHS, Id: F32 GM086038
  • Agency: NIGMS NIH HHS, Id: T32 GM008759
  • Agency: NIGMS NIH HHS, Id: R01 GM051312
  • Agency: NCRR NIH HHS, Id: U54 RR022220
  • Agency: NCRR NIH HHS, Id: P41 RR011823
  • Agency: Canadian Institutes of Health Research, Id: MOP-64404
  • Agency: NHGRI NIH HHS, Id: R01 HG003039
  • Agency: NIGMS NIH HHS, Id: R01 GM051312-16
  • Agency: NIGMS NIH HHS, Id: GM51312
  • Agency: NIGMS NIH HHS, Id: R01 GM062427

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