• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

CREST maps somatic structural variation in cancer genomes with base-pair resolution.

We developed 'clipping reveals structure' (CREST), an algorithm that uses next-generation sequencing reads with partial alignments to a reference genome to directly map structural variations at the nucleotide level of resolution. Application of CREST to whole-genome sequencing data from five pediatric T-lineage acute lymphoblastic leukemias (T-ALLs) and a human melanoma cell line, COLO-829, identified 160 somatic structural variations. Experimental validation exceeded 80%, demonstrating that CREST had a high predictive accuracy.

Pubmed ID: 21666668

Authors

  • Wang J
  • Mullighan CG
  • Easton J
  • Roberts S
  • Heatley SL
  • Ma J
  • Rusch MC
  • Chen K
  • Harris CC
  • Ding L
  • Holmfeldt L
  • Payne-Turner D
  • Fan X
  • Wei L
  • Zhao D
  • Obenauer JC
  • Naeve C
  • Mardis ER
  • Wilson RK
  • Downing JR
  • Zhang J

Journal

Nature methods

Publication Data

August 29, 2011

Associated Grants

  • Agency: NCI NIH HHS, Id: P30 CA021765
  • Agency: NCI NIH HHS, Id: P30 CA021765

Mesh Terms

  • Algorithms
  • Animals
  • Base Pair Mismatch
  • DNA, Neoplasm
  • Genome
  • Humans
  • Neoplasms
  • Polymorphism, Single Nucleotide
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Software