Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A function for cyclin D1 in DNA repair uncovered by protein interactome analyses in human cancers.

Nature | Jun 8, 2011

Cyclin D1 is a component of the core cell cycle machinery. Abnormally high levels of cyclin D1 are detected in many human cancer types. To elucidate the molecular functions of cyclin D1 in human cancers, we performed a proteomic screen for cyclin D1 protein partners in several types of human tumours. Analyses of cyclin D1 interactors revealed a network of DNA repair proteins, including RAD51, a recombinase that drives the homologous recombination process. We found that cyclin D1 directly binds RAD51, and that cyclin D1-RAD51 interaction is induced by radiation. Like RAD51, cyclin D1 is recruited to DNA damage sites in a BRCA2-dependent fashion. Reduction of cyclin D1 levels in human cancer cells impaired recruitment of RAD51 to damaged DNA, impeded the homologous recombination-mediated DNA repair, and increased sensitivity of cells to radiation in vitro and in vivo. This effect was seen in cancer cells lacking the retinoblastoma protein, which do not require D-cyclins for proliferation. These findings reveal an unexpected function of a core cell cycle protein in DNA repair and suggest that targeting cyclin D1 may be beneficial also in retinoblastoma-negative cancers which are currently thought to be unaffected by cyclin D1 inhibition.

Pubmed ID: 21654808 RIS Download

Mesh terms: Animals | Cell Line, Tumor | Comet Assay | Cyclin D1 | DNA Damage | DNA Repair | HeLa Cells | Humans | Mice | Neoplasms | Protein Binding | Protein Interaction Mapping | Rad51 Recombinase | Radiation, Ionizing | Recombination, Genetic | Retinoblastoma Protein

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA083688-10
  • Agency: NCI NIH HHS, Id: R01 CA083688
  • Agency: NCI NIH HHS, Id: P01 CA109901
  • Agency: NCI NIH HHS, Id: P01 CA080111
  • Agency: NCI NIH HHS, Id: R01 CA138804
  • Agency: NCI NIH HHS, Id: P01 CA109901-07
  • Agency: NIEHS NIH HHS, Id: Z01 ES065089
  • Agency: Intramural NIH HHS, Id: Z01 ES065089-11
  • Agency: NCI NIH HHS, Id: P01 CA080111-12
  • Agency: NCI NIH HHS, Id: R01 CA138804-02
  • Agency: NIAID NIH HHS, Id: P30 AI060354

OMIM (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.