Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators.

Developmental cell | May 17, 2011

Hematopoietic differentiation critically depends on combinations of transcriptional regulators controlling the development of individual lineages. Here, we report the genome-wide binding sites for the five key hematopoietic transcription factors--GATA1, GATA2, RUNX1, FLI1, and TAL1/SCL--in primary human megakaryocytes. Statistical analysis of the 17,263 regions bound by at least one factor demonstrated that simultaneous binding by all five factors was the most enriched pattern and often occurred near known hematopoietic regulators. Eight genes not previously appreciated to function in hematopoiesis that were bound by all five factors were shown to be essential for thrombocyte and/or erythroid development in zebrafish. Moreover, one of these genes encoding the PDZK1IP1 protein shared transcriptional enhancer elements with the blood stem cell regulator TAL1/SCL. Multifactor ChIP-Seq analysis in primary human cells coupled with a high-throughput in vivo perturbation screen therefore offers a powerful strategy to identify essential regulators of complex mammalian differentiation processes.

Pubmed ID: 21571218 RIS Download

Mesh terms: Basic Helix-Loop-Helix Transcription Factors | Binding Sites | Cell Differentiation | Cells, Cultured | Core Binding Factor Alpha 2 Subunit | GATA1 Transcription Factor | GATA2 Transcription Factor | Genome, Human | Humans | Megakaryocytes | Oligonucleotide Array Sequence Analysis | Proto-Oncogene Protein c-fli-1 | Proto-Oncogene Proteins

Research resources used in this publication

None found

Research tools detected in this publication

Data used in this publication

None found

Associated grants

  • Agency: Wellcome Trust, Id: 082597/Z/07/Z
  • Agency: Wellcome Trust, Id: 077037/Z/05/Z
  • Agency: Wellcome Trust, Id: 077047/Z/05/Z
  • Agency: National Centre for the Replacement, Refinement and Reduction of Animals in Research, Id: G0900729/1
  • Agency: Medical Research Council, Id: G0800784
  • Agency: Medical Research Council, Id: G0900951
  • Agency: Medical Research Council, Id: RG/09/012/28096
  • Agency: British Heart Foundation, Id: G0900951(91754)
  • Agency: Medical Research Council, Id:

ZFIN (Data, Gene Expression)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Gene Expression Omnibus

A public functional genomics data repository supporting MIAME-compliant data submissions. Tools are provided to help users query and download experiments and curated gene expression profiles. These data include microarray-based experiments measuring the abundance of mRNA, genomic DNA, and protein molecules, as well as non-array-based technologies such as serial analysis of gene expression (SAGE) and mass spectrometry proteomic technology. Array- and sequence-based data are accepted.

tool

View all literature mentions