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Genetic analysis of basophil function in vivo.

Nature immunology | Jun 18, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21552267

Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.

Pubmed ID: 21552267 RIS Download

Mesh terms: Animals | Basophils | CD4-Positive T-Lymphocytes | Female | Flow Cytometry | Green Fluorescent Proteins | Helminthiasis, Animal | Host-Parasite Interactions | Humans | Interleukin-13 | Interleukin-4 | Liver | Lung | Lung Diseases, Parasitic | Male | Mice | Mice, Inbred C57BL | Mice, Knockout | Mice, Transgenic | Microscopy, Fluorescence, Multiphoton | Nippostrongylus | Schistosoma mansoni | Strongylida Infections

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Associated grants

  • Agency: NIAID NIH HHS, Id: AI026918
  • Agency: NIAID NIH HHS, Id: AI077439
  • Agency: NIAID NIH HHS, Id: R37 AI026918
  • Agency: NIAID NIH HHS, Id: R37 AI026918-24
  • Agency: NICHD NIH HHS, Id: T32 HD044331
  • Agency: NIAID NIH HHS, Id: U19 AI077439
  • Agency: NIAID NIH HHS, Id: U19 AI077439-05
  • Agency: Howard Hughes Medical Institute, Id:

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