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Genetic analysis of basophil function in vivo.

Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.

Pubmed ID: 21552267

Authors

  • Sullivan BM
  • Liang HE
  • Bando JK
  • Wu D
  • Cheng LE
  • McKerrow JK
  • Allen CD
  • Locksley RM

Journal

Nature immunology

Publication Data

June 18, 2011

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI026918
  • Agency: NIAID NIH HHS, Id: AI077439
  • Agency: NIAID NIH HHS, Id: R37 AI026918
  • Agency: NIAID NIH HHS, Id: R37 AI026918-24
  • Agency: NICHD NIH HHS, Id: T32 HD044331
  • Agency: NIAID NIH HHS, Id: U19 AI077439
  • Agency: NIAID NIH HHS, Id: U19 AI077439-05
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Animals
  • Basophils
  • CD4-Positive T-Lymphocytes
  • Female
  • Flow Cytometry
  • Green Fluorescent Proteins
  • Helminthiasis, Animal
  • Host-Parasite Interactions
  • Humans
  • Interleukin-13
  • Interleukin-4
  • Liver
  • Lung
  • Lung Diseases, Parasitic
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Microscopy, Fluorescence, Multiphoton
  • Nippostrongylus
  • Schistosoma mansoni
  • Strongylida Infections