Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase.

Accurate genome segregation depends on cohesion mechanisms that link duplicated sister chromatids, thereby allowing their tension-dependent biorientation in metaphase. In Saccharomyces cerevisiae, cohesion is established during DNA replication when Eco1 acetylates the cohesin subunit Smc3. Cohesion establishment is restricted to S phase of the cell cycle, but the molecular basis of this regulation is unknown. Here, we show that Eco1 is negatively regulated by the protein kinase Cdk1. Phosphorylation of Eco1 after S phase targets it to SCF(Cdc4) for ubiquitination and subsequent degradation. A nonphosphorylatable mutant of Eco1 establishes cohesion after DNA replication, suggesting that Cdk1-dependent phosphorylation of Eco1 is a key factor limiting establishment to S phase. We also show that deregulation of Eco1 results in chromosome separation defects in anaphase. We conclude that this regulatory mechanism helps optimize the level of sister chromatid cohesion, ensuring a robust and efficient anaphase.

Pubmed ID: 21549314


  • Lyons NA
  • Morgan DO


Molecular cell

Publication Data

May 6, 2011

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM069901
  • Agency: NIGMS NIH HHS, Id: R01 GM069901
  • Agency: NIGMS NIH HHS, Id: R01 GM069901-08

Mesh Terms

  • Acetylation
  • Acetyltransferases
  • Anaphase
  • Blotting, Western
  • CDC2 Protein Kinase
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation
  • DNA Replication
  • F-Box Proteins
  • Mutation
  • Nuclear Proteins
  • Phosphorylation
  • S Phase
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Time Factors
  • Ubiquitin-Protein Ligases
  • Ubiquitination