• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.

Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). We also found that an actin signaling protein, Toca-1, enhances Oct4 reactivation by regulating nuclear actin polymerization. Toca-1 overexpression has an effect on the chromatin state of transplanted nuclei, including the enhanced binding of nuclear actin to gene regulatory regions. This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner.

Pubmed ID: 21536734

Authors

  • Miyamoto K
  • Pasque V
  • Jullien J
  • Gurdon JB

Journal

Genes & development

Publication Data

May 1, 2011

Associated Grants

  • Agency: Wellcome Trust, Id: 081277
  • Agency: Wellcome Trust, Id: 081277
  • Agency: Wellcome Trust, Id: RG54943

Mesh Terms

  • Actins
  • Animals
  • Carrier Proteins
  • Cell Line
  • Cell Nucleus
  • Cellular Reprogramming
  • Chromatin Assembly and Disassembly
  • Gene Expression Regulation, Developmental
  • Mice
  • Octamer Transcription Factor-3
  • Oocytes
  • Polymerization
  • Signal Transduction
  • Xenopus
  • Xenopus Proteins