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Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.

Genes & development | May 1, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21536734

Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). We also found that an actin signaling protein, Toca-1, enhances Oct4 reactivation by regulating nuclear actin polymerization. Toca-1 overexpression has an effect on the chromatin state of transplanted nuclei, including the enhanced binding of nuclear actin to gene regulatory regions. This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner.

Pubmed ID: 21536734 RIS Download

Mesh terms: Actins | Animals | Carrier Proteins | Cell Line | Cell Nucleus | Cellular Reprogramming | Chromatin Assembly and Disassembly | Gene Expression Regulation, Developmental | Mice | Octamer Transcription Factor-3 | Oocytes | Polymerization | Signal Transduction | Xenopus | Xenopus Proteins

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Associated grants

  • Agency: Wellcome Trust, Id: 081277
  • Agency: Wellcome Trust, Id: 081277
  • Agency: Wellcome Trust, Id: RG54943

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