Our hosting provider will be performing UPS maintenance on Tuesday, Oct 25, 2016 between 8 AM and 5 PM PDT. SciCrunch searching services will be down during this time.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity.


The facial branchiomotor neurons (FBMNs) undergo a characteristic tangential migration in the vertebrate hindbrain. We previously used a morpholino knockdown approach to reveal that zebrafish prickle1b (pk1b) is required for this migration. Here we report that FBMN migration is also blocked in a pk1b mutant with a disruption in the consensus farnesylation motif. We confirmed that this lipid modification is required during FBMN migration by disrupting the function of farnesyl biosynthetic enzymes. Furthermore, farnesylation of a tagged Pk1b is required for its nuclear localization. Using a unique rescue approach, we have demonstrated that Pk1b nuclear localization and farnesylation are required during FBMN migration. Our data suggest that Pk1b acts at least partially independently of core planar cell polarity molecules at the plasma membrane, and might instead be acting at the nucleus. We also found that the neuronal transcriptional silencer REST is necessary for FBMN migration, and we provide evidence that interaction between Pk1b and REST is required during this process. Finally, we demonstrate that REST protein, which is normally localized in the nuclei of migrating FBMNs, is depleted from the nuclei of Pk1b-deficient neurons. We conclude that farnesylation-dependent nuclear localization of Pk1b is required to regulate REST localization and thus FBMN migration.

Pubmed ID: 21521740


  • Mapp OM
  • Walsh GS
  • Moens CB
  • Tada M
  • Prince VE


Development (Cambridge, England)

Publication Data

May 27, 2011

Associated Grants

  • Agency: NICHD NIH HHS, Id: 5P30HD054275
  • Agency: NIGMS NIH HHS, Id: 5T32GM007197
  • Agency: Medical Research Council, Id: G0401126
  • Agency: NICHD NIH HHS, Id: R01HD037909
  • Agency: NCRR NIH HHS, Id: UL1RR024999
  • Agency: Howard Hughes Medical Institute, Id:
  • Agency: Medical Research Council, Id:

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Carrier Proteins
  • Cell Movement
  • Cell Nucleus
  • DNA, Complementary
  • Farnesyltranstransferase
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Hydroxymethylglutaryl CoA Reductases
  • LIM Domain Proteins
  • Models, Biological
  • Molecular Sequence Data
  • Motor Neurons
  • Mutation
  • Neurogenesis
  • Protein Prenylation
  • Repressor Proteins
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Zebrafish
  • Zebrafish Proteins