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Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation.

Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partially complements loss of budding yeast TVP23 function and a Populus ECH complements the Arabidopsis ech mutant, suggesting functional conservation of the genes. Compared with wild-type, the Arabidopsis ech mutant exhibits severely perturbed cell elongation as well as defects in TGN structure and function, manifested by the reduced association between Golgi bodies and TGN as well as mislocalization of several TGN-localized proteins including vacuolar H(+)-ATPase subunit a1 (VHA-a1). Strikingly, ech is defective in secretory trafficking, whereas endocytosis appears unaffected in the mutant. Some aspects of the ech mutant phenotype can be phenocopied by treatment with a specific inhibitor of vacuolar H(+)-ATPases, concanamycin A, indicating that mislocalization of VHA-a1 may account for part of the defects in ech. Hence, ECH is an evolutionarily conserved component of the TGN with a central role in TGN structure and function.

Pubmed ID: 21512130

Authors

  • Gendre D
  • Oh J
  • Boutté Y
  • Best JG
  • Samuels L
  • Nilsson R
  • Uemura T
  • Marchant A
  • Bennett MJ
  • Grebe M
  • Bhalerao RP

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

May 10, 2011

Associated Grants

  • Agency: Biotechnology and Biological Sciences Research Council, Id: BB/G023972/1

Mesh Terms

  • Amino Acid Sequence
  • Arabidopsis
  • Arabidopsis Proteins
  • Base Sequence
  • Cell Compartmentation
  • Cell Shape
  • DNA, Plant
  • Evolution, Molecular
  • Genes, Plant
  • Genetic Complementation Test
  • Macrolides
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Phylogeny
  • Plants, Genetically Modified
  • Saccharomyces cerevisiae
  • Sequence Homology, Amino Acid
  • Vesicular Transport Proteins
  • trans-Golgi Network