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Congenital bone marrow failure in DNA-PKcs mutant mice associated with deficiencies in DNA repair.

The nonhomologous end-joining (NHEJ) pathway is essential for radioresistance and lymphocyte-specific V(D)J (variable [diversity] joining) recombination. Defects in NHEJ also impair hematopoietic stem cell (HSC) activity with age but do not affect the initial establishment of HSC reserves. In this paper, we report that, in contrast to deoxyribonucleic acid (DNA)-dependent protein kinase catalytic subunit (DNA-PKcs)-null mice, knockin mice with the DNA-PKcs(3A/3A) allele, which codes for three alanine substitutions at the mouse Thr2605 phosphorylation cluster, die prematurely because of congenital bone marrow failure. Impaired proliferation of DNA-PKcs(3A/3A) HSCs is caused by excessive DNA damage and p53-dependent apoptosis. In addition, increased apoptosis in the intestinal crypt and epidermal hyperpigmentation indicate the presence of elevated genotoxic stress and p53 activation. Analysis of embryonic fibroblasts further reveals that DNA-PKcs(3A/3A) cells are hypersensitive to DNA cross-linking agents and are defective in both homologous recombination and the Fanconi anemia DNA damage response pathways. We conclude that phosphorylation of DNA-PKcs is essential for the normal activation of multiple DNA repair pathways, which in turn is critical for the maintenance of diverse populations of tissue stem cells in mice.

Pubmed ID: 21482716 RIS Download

Mesh terms: Animals | Apoptosis | Cells, Cultured | Cross-Linking Reagents | DNA Damage | DNA Repair | DNA-Activated Protein Kinase | DNA-Binding Proteins | Fanconi Anemia | Fibroblasts | Gene Knock-In Techniques | Hematopoiesis | Hematopoietic Stem Cells | Hyperpigmentation | Mice | Mice, Knockout | Mutation | Nuclear Proteins | Recombination, Genetic | Tumor Suppressor Protein p53

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Associated grants

  • Agency: NCI NIH HHS, Id: CA120099
  • Agency: NCI NIH HHS, Id: R37 CA050519
  • Agency: NCI NIH HHS, Id: K01 CA120099
  • Agency: NCI NIH HHS, Id: CA50519
  • Agency: NCI NIH HHS, Id: R01 CA050519

Mouse Genome Informatics (Data, Gene Annotation)

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