• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Monoallelic deletion of the microRNA biogenesis gene Dgcr8 produces deficits in the development of excitatory synaptic transmission in the prefrontal cortex.

BACKGROUND: Neuronal phenotypes associated with hemizygosity of individual genes within the 22q11.2 deletion syndrome locus hold potential towards understanding the pathogenesis of schizophrenia and autism. Included among these genes is Dgcr8, which encodes an RNA-binding protein required for microRNA biogenesis. Dgcr8 haploinsufficient mice (Dgcr8+/-) have reduced expression of microRNAs in brain and display cognitive deficits, but how microRNA deficiency affects the development and function of neurons in the cerebral cortex is not fully understood. RESULTS: In this study, we show that Dgcr8+/- mice display reduced expression of a subset of microRNAs in the prefrontal cortex, a deficit that emerges over postnatal development. Layer V pyramidal neurons in the medial prefrontal cortex of Dgcr8+/- mice have altered electrical properties, decreased complexity of basal dendrites, and reduced excitatory synaptic transmission. CONCLUSIONS: These findings demonstrate that precise microRNA expression is critical for the postnatal development of prefrontal cortical circuitry. Similar defects in neuronal maturation resulting from microRNA deficiency could represent endophenotypes of certain neuropsychiatric diseases of developmental onset.

Pubmed ID: 21466685


  • Schofield CM
  • Hsu R
  • Barker AJ
  • Gertz CC
  • Blelloch R
  • Ullian EM


Neural development

Publication Data

April 27, 2011

Associated Grants

  • Agency: NIMH NIH HHS, Id: R21MH083090

Mesh Terms

  • Animals
  • Brain
  • Dendrites
  • Electrophysiological Phenomena
  • Excitatory Postsynaptic Potentials
  • Image Processing, Computer-Assisted
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • MicroRNAs
  • Neurons
  • Patch-Clamp Techniques
  • Prefrontal Cortex
  • Proteins
  • Pyramidal Cells
  • RNA-Binding Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Synaptic Transmission