Preparing your results

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The Caenorhabditis elegans JIP3 protein UNC-16 functions as an adaptor to link kinesin-1 with cytoplasmic dynein.

Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show that Caenorhabditis elegans kinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both kinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo molecules in vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein.

Pubmed ID: 21307258


  • Arimoto M
  • Koushika SP
  • Choudhary BC
  • Li C
  • Matsumoto K
  • Hisamoto N


The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

February 9, 2011

Associated Grants


Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • COS Cells
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Cercopithecus aethiops
  • Cytoplasmic Dyneins
  • Dyneins
  • Gene Expression Regulation
  • Immunoprecipitation
  • Kinesin
  • Luminescent Proteins
  • Mechanoreceptors
  • Membrane Proteins
  • Microinjections
  • Presynaptic Terminals
  • Protein Binding
  • Protein Transport
  • Transfection