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A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms.

Repressor activator protein 1 (RAP1) is the most highly conserved telomere protein. It is involved in protecting chromosome ends in fission yeast and promoting gene silencing in Saccharomyces cerevisiae, whereas it represses homology-directed recombination at telomeres in mammals. To understand how RAP1 has such diverse functions at telomeres, we solved the crystal or solution structures of the RAP1 C-terminal (RCT) domains of RAP1 from multiple organisms in complex with their respective protein-binding partners. Our analysis establishes RAP1(RCT) as an evolutionarily conserved protein-protein interaction module. In mammalian and fission yeast cells, this module interacts with TRF2 and Taz1, respectively, targeting RAP1 to chromosome ends for telomere protection. In contrast, S. cerevisiae RAP1 uses its RCT domain to recruit Sir3 to telomeres to mediate gene silencing. Together, our results show that, depending on the organism, the evolutionarily conserved RAP1 RCT motif has diverse functional roles at telomeres.

Pubmed ID: 21217703 RIS Download

Mesh terms: Amino Acid Motifs | Amino Acid Sequence | Animals | Cells, Cultured | Crystallography, X-Ray | Fungal Proteins | HeLa Cells | Humans | Models, Molecular | Molecular Sequence Data | Mutation | Nuclear Magnetic Resonance, Biomolecular | Protein Binding | Protein Interaction Domains and Motifs | Saccharomycetales | Schizosaccharomyces | Telomere | Telomere-Binding Proteins | Telomeric Repeat Binding Protein 2

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Associated grants

  • Agency: NIA NIH HHS, Id: R01 AG028888
  • Agency: NCI NIH HHS, Id: R01 CA129037
  • Agency: NIGMS NIH HHS, Id: R01 GM083015

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