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Yeast Sen1 helicase protects the genome from transcription-associated instability.

Molecular cell | Jan 7, 2011

http://www.ncbi.nlm.nih.gov/pubmed/21211720

Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability.

Pubmed ID: 21211720 RIS Download

Mesh terms: DNA Damage | DNA Helicases | DNA Repair | Genomic Instability | Nucleic Acid Conformation | Nucleic Acid Hybridization | Protein Structure, Tertiary | RNA Helicases | Recombination, Genetic | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription, Genetic

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Associated grants

  • Agency: Wellcome Trust, Id:

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