Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

14-3-3sigma regulates B-cell homeostasis through stabilization of FOXO1.

http://www.ncbi.nlm.nih.gov/pubmed/21205887

14-3-3σ regulates cytokinesis and cell cycle arrest induced by DNA damage but its role in the immune system is unknown. Using gene-targeted 14-3-3σ-deficient (i.e., KO) mice, we studied the role of 14-3-3σ in B-cell functions. Total numbers of B cells were reduced by spontaneous apoptosis of peripheral B cells. Upon B-cell antigen receptor engagement in vitro, KO B cells did not proliferate properly or up-regulate CD86. In response to T cell-independent antigens, KO B cells showed poor secretion of antigen-specific IgM. This deficit led to increased lethality of KO mice after vesicular stomatitis virus infection. KO B cells showed elevated total FOXO transcriptional activity but also increased FOXO1 degradation. Coimmunoprecipitation revealed that endogenous 14-3-3σ protein formed a complex with FOXO1 protein. Our results suggest that 14-3-3σ maintains FOXO1 at a consistent level critical for normal B-cell antigen receptor signaling and B-cell survival.

Pubmed ID: 21205887 RIS Download

Mesh terms: 14-3-3 Proteins | Adoptive Transfer | Animals | Antigens | Apoptosis | B-Lymphocytes | Blotting, Western | Cell Proliferation | Cells, Cultured | Enzyme-Linked Immunosorbent Assay | Female | Ficoll | Forkhead Transcription Factors | Homeostasis | Immunoglobulin G | Immunoglobulin M | Male | Mice | Mice, Inbred BALB C | Mice, Inbred C57BL | Mice, Knockout | Protein Binding | Receptors, Antigen, B-Cell | Reverse Transcriptase Polymerase Chain Reaction | Trinitrobenzenes

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.