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Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1.

The anaphase-promoting complex/cyclosome (APC/C) is a 22S ubiquitin ligase complex that initiates chromosome segregation and mitotic exit. We have used biochemical and electron microscopic analyses of Saccharomyces cerevisiae and human APC/C to address how the APC/C subunit Doc1 contributes to recruitment and processive ubiquitylation of APC/C substrates, and to understand how APC/C monomers interact to form a 36S dimeric form. We show that Doc1 interacts with Cdc27, Cdc16 and Apc1 and is located in the vicinity of the cullin-RING module Apc2-Apc11 in the inner cavity of the APC/C. Substrate proteins also bind in the inner cavity, in close proximity to Doc1 and the coactivator Cdh1, and induce conformational changes in Apc2-Apc11. Our results suggest that substrates are recruited to the APC/C by binding to a bipartite substrate receptor composed of a coactivator protein and Doc1.

Pubmed ID: 21186364 RIS Download

Mesh terms: Anaphase-Promoting Complex-Cyclosome | Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome | Cadherins | Cdh1 Proteins | Cell Cycle Proteins | Cytokines | Humans | Intracellular Signaling Peptides and Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Ubiquitin-Protein Ligase Complexes | Ubiquitin-Protein Ligases | Ubiquitination

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Associated grants

  • Agency: Austrian Science Fund FWF, Id: F 3407

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SGD

A curated database that provides comprehensive integrated biological information for Saccharomyces cerevisiae along with search and analysis tools to explore these data. SGD allows researchers to discover functional relationships between sequence and gene products in fungi and higher organisms. The SGD also maintains the S. cerevisiae Gene Name Registry, a complete list of all gene names used in S. cerevisiae which includes a set of general guidelines to gene naming. Protein Page provides basic protein information calculated from the predicted sequence and contains links to a variety of secondary structure and tertiary structure resources. Yeast Biochemical Pathways allows users to view and search for biochemical reactions and pathways that occur in S. cerevisiae as well as map expression data onto the biochemical pathways. Literature citations are provided where available.

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