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The yeast Arf GTPase-activating protein Age1 is regulated by phospholipase D for post-Golgi vesicular transport.

Vesicular transport shuttles cargo among intracellular compartments. Several stages of vesicular transport are mediated by the small GTPase Arf, which is controlled in a cycle of GTP binding and hydrolysis by Arf guanine-nucleotide exchange factors and Arf GTPase-activating proteins (ArfGAPs), respectively. In budding yeast the Age2 + Gcs1 ArfGAP pair facilitates post-Golgi transport. We have found the AGE1 gene, encoding another ArfGAP, can in high gene-copy number alleviate the temperature sensitivity of cells carrying mutations affecting the Age2 + Gcs1 ArfGAP pair. Moreover, increased AGE1 gene dosage compensates for the complete absence of the otherwise essential Age2 + Gcs1 ArfGAP pair. Increased dosage of SFH2, encoding a phosphatidylinositol transfer protein, also allows cell growth in the absence of the Age2 + Gcs1 pair, but good growth in this situation requires Age1. The ability of Age1 to overcome the need for Age2 + Gcs1 depends on phospholipase D activity that regulates lipid composition. We show by direct assessment of Age1 ArfGAP activity that Age1 is regulated by lipid composition and can provide ArfGAP function for post-Golgi transport.

Pubmed ID: 21135091


  • Benjamin JJ
  • Poon PP
  • Lewis SM
  • Auger A
  • Wong TA
  • Singer RA
  • Johnston GC


The Journal of biological chemistry

Publication Data

February 18, 2011

Associated Grants

  • Agency: Canadian Institutes of Health Research, Id: MOP-64293

Mesh Terms

  • ADP-Ribosylation Factors
  • Biological Transport
  • DNA-Binding Proteins
  • GTPase-Activating Proteins
  • Gene Dosage
  • Golgi Apparatus
  • Membrane Lipids
  • Phospholipase D
  • Phospholipid Transfer Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transport Vesicles