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The yeast Arf GTPase-activating protein Age1 is regulated by phospholipase D for post-Golgi vesicular transport.

http://www.ncbi.nlm.nih.gov/pubmed/21135091

Vesicular transport shuttles cargo among intracellular compartments. Several stages of vesicular transport are mediated by the small GTPase Arf, which is controlled in a cycle of GTP binding and hydrolysis by Arf guanine-nucleotide exchange factors and Arf GTPase-activating proteins (ArfGAPs), respectively. In budding yeast the Age2 + Gcs1 ArfGAP pair facilitates post-Golgi transport. We have found the AGE1 gene, encoding another ArfGAP, can in high gene-copy number alleviate the temperature sensitivity of cells carrying mutations affecting the Age2 + Gcs1 ArfGAP pair. Moreover, increased AGE1 gene dosage compensates for the complete absence of the otherwise essential Age2 + Gcs1 ArfGAP pair. Increased dosage of SFH2, encoding a phosphatidylinositol transfer protein, also allows cell growth in the absence of the Age2 + Gcs1 pair, but good growth in this situation requires Age1. The ability of Age1 to overcome the need for Age2 + Gcs1 depends on phospholipase D activity that regulates lipid composition. We show by direct assessment of Age1 ArfGAP activity that Age1 is regulated by lipid composition and can provide ArfGAP function for post-Golgi transport.

Pubmed ID: 21135091 RIS Download

Mesh terms: ADP-Ribosylation Factors | Biological Transport | DNA-Binding Proteins | GTPase-Activating Proteins | Gene Dosage | Golgi Apparatus | Membrane Lipids | Phospholipase D | Phospholipid Transfer Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transport Vesicles

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Associated grants

  • Agency: Canadian Institutes of Health Research, Id: MOP-64293

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