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Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p.

Regulated gene expression makes an important contribution to cell cycle control mechanisms. In fission yeast, a group of genes is coordinately expressed during a late stage of the cell cycle (M phase and cytokinesis) that is controlled by common cis-acting promoter motifs named pombe cell cycle boxes (PCBs), which are bound by a trans-acting transcription factor complex, PCB binding factor (PBF). PBF contains at least three transcription factors, a MADS box protein Mbx1p and two forkhead transcription factors, Sep1p and Fkh2p. Here we show that the fission yeast Cdc14p-like phosphatase Clp1p (Flp1p) controls M-G1 specific gene expression through PBF. Clp1p binds in vivo both to Mbx1p, a MADS box-like transcription factor, and to the promoters of genes transcribed at this cell cycle time. Because Clp1p dephosphorylates Mbx1p in vitro, and is required for Mbx1p cell cycle-specific dephosphorylation in vivo, our observations suggest that Clp1p controls cell cycle-specific gene expression through binding to and dephosphorylating Mbx1p.

Pubmed ID: 21098641

Authors

  • Papadopoulou K
  • Chen JS
  • Mead E
  • Feoktistova A
  • Petit C
  • Agarwal M
  • Jamal M
  • Malik A
  • Spanos A
  • Sedgwick SG
  • Karagiannis J
  • Balasubramanian MK
  • Gould KL
  • McInerny CJ

Journal

Journal of cell science

Publication Data

December 15, 2010

Associated Grants

None

Mesh Terms

  • Cell Cycle
  • Cell Cycle Proteins
  • G1 Phase
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Mitosis
  • Models, Genetic
  • Phosphoprotein Phosphatases
  • Phosphorylation
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Tyrosine Phosphatases
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins