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Bassoon and the synaptic ribbon organize Ca²+ channels and vesicles to add release sites and promote refilling.

Neuron | Nov 18, 2010

At the presynaptic active zone, Ca²+ influx triggers fusion of synaptic vesicles. It is not well understood how Ca²+ channel clustering and synaptic vesicle docking are organized. Here, we studied structure and function of hair cell ribbon synapses following genetic disruption of the presynaptic scaffold protein Bassoon. Mutant synapses--mostly lacking the ribbon--showed a reduction in membrane-proximal vesicles, with ribbonless synapses affected more than ribbon-occupied synapses. Ca²+ channels were also fewer at mutant synapses and appeared in abnormally shaped clusters. Ribbon absence reduced Ca²+ channel numbers at mutant and wild-type synapses. Fast and sustained exocytosis was reduced, notwithstanding normal coupling of the remaining Ca²+ channels to exocytosis. In vitro recordings revealed a slight impairment of vesicle replenishment. Mechanistic modeling of the in vivo data independently supported morphological and functional in vitro findings. We conclude that Bassoon and the ribbon (1) create a large number of release sites by organizing Ca²+ channels and vesicles, and (2) promote vesicle replenishment.

Pubmed ID: 21092861 RIS Download

Mesh terms: Animals | Calcium Channels | Excitatory Postsynaptic Potentials | Exocytosis | Mice | Mice, 129 Strain | Mice, Inbred C57BL | Mice, Transgenic | Nerve Tissue Proteins | Neuronal Plasticity | Synapses | Synaptic Vesicles

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Associated grants

  • Agency: NIAID NIH HHS, Id: T32 AI007260
  • Agency: NIAID NIH HHS, Id: T32 AI07260
  • Agency: NHLBI NIH HHS, Id: R01 HL087120
  • Agency: NIDCD NIH HHS, Id: R01 DC009433-04
  • Agency: NIDCD NIH HHS, Id: R01 DC009433
  • Agency: NIDCD NIH HHS, Id: P30 DC010362
  • Agency: NIAID NIH HHS, Id: T32 AI007260-20
  • Agency: NIDCD NIH HHS, Id: DC009433
  • Agency: NHLBI NIH HHS, Id: HL087120
  • Agency: NHLBI NIH HHS, Id: R01 HL087120-03
  • Agency: NIDCD NIH HHS, Id: P30 DC005209
  • Agency: NIDCD NIH HHS, Id: R55 DC009433

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