Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.

Neuron | Nov 18, 2010

http://www.ncbi.nlm.nih.gov/pubmed/21092859

Coordinated migration of newly born neurons to their prospective target laminae is a prerequisite for neural circuit assembly in the developing brain. The evolutionarily conserved LIS1/NDEL1 complex is essential for neuronal migration in the mammalian cerebral cortex. The cytoplasmic nature of LIS1 and NDEL1 proteins suggest that they regulate neuronal migration cell autonomously. Here, we extend mosaic analysis with double markers (MADM) to mouse chromosome 11 where Lis1, Ndel1, and 14-3-3ɛ (encoding a LIS1/NDEL1 signaling partner) are located. Analyses of sparse and uniquely labeled mutant cells in mosaic animals reveal distinct cell-autonomous functions for these three genes. Lis1 regulates neuronal migration efficiency in a dose-dependent manner, while Ndel1 is essential for a specific, previously uncharacterized, late step of neuronal migration: entry into the target lamina. Comparisons with previous genetic perturbations of Lis1 and Ndel1 also suggest a surprising degree of cell-nonautonomous function for these proteins in regulating neuronal migration.

Pubmed ID: 21092859 RIS Download

Mesh terms: 1-Alkyl-2-acetylglycerophosphocholine Esterase | Animals | Animals, Newborn | Astrocytes | Carrier Proteins | Cell Movement | Cerebral Cortex | Female | Male | Mice | Mice, Transgenic | Microtubule-Associated Proteins | Mosaicism | Neurons

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NICHD NIH HHS, Id: HD047380
  • Agency: NINDS NIH HHS, Id: NS041310
  • Agency: NINDS NIH HHS, Id: NS050835
  • Agency: NINDS NIH HHS, Id: R01 NS050835
  • Agency: NINDS NIH HHS, Id: R01 NS050835-08
  • Agency: Howard Hughes Medical Institute, Id:

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.