Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124.
The orphan G protein-coupled receptor (GPCR) GPR124/tumor endothelial marker 5 is highly expressed in central nervous system (CNS) endothelium. Here, we show that complete null or endothelial-specific GPR124 deletion resulted in embryonic lethality from CNS-specific angiogenesis arrest in forebrain and neural tube. Conversely, GPR124 overexpression throughout all adult vascular beds produced CNS-specific hyperproliferative vascular malformations. In vivo, GPR124 functioned cell-autonomously in endothelium to regulate sprouting, migration, and developmental expression of the blood-brain barrier marker Glut1, whereas in vitro, GPR124 mediated Cdc42-dependent directional migration to forebrain-derived, vascular endothelial growth factor-independent cues. Our results demonstrate CNS-specific angiogenesis regulation by an endothelial receptor and illuminate functions of the poorly understood adhesion GPCR subfamily. Further, the functional tropism of GPR124 marks this receptor as a therapeutic target for CNS-related vascular pathologies.
Pubmed ID: 21071672 RIS Download
Animals | Blood Vessels | Blood-Brain Barrier | Cell Movement | Embryonic Development | Endothelial Cells | Endothelium, Vascular | Gene Deletion | Glucose Transporter Type 1 | Mesencephalon | Mice | Mice, Knockout | Mice, Transgenic | Neovascularization, Physiologic | Neural Tube | Prosencephalon | Receptors, G-Protein-Coupled | Rhombencephalon | Telencephalon