Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty liver disease or metabolic syndrome.

Journal of lipid research | Feb 17, 2011

PNPLA3 (adiponutrin, calcium-independent phospholipase A(2) epsilon [iPLA(2)ε]) is an adipose-enriched, nutritionally regulated protein that belongs to the patatin-like phospholipase domain containing (PNPLA) family of lipid metabolizing proteins. Genetic variations in the human PNPLA3 gene (i.e., the rs738409 I148M allele) has been strongly and repeatedly associated with fatty liver disease. Although human PNPLA3 has triacylglycerol (TAG) hydrolase and transacylase activities in vitro, its in vivo function and physiological relevance remain controversial. The objective of this study was to determine the metabolic consequences of global targeted deletion of the Pnpla3 gene in mice. We found that Pnpla3 mRNA expression is altered in adipose tissue and liver in response to acute and chronic nutritional challenges. However, global targeted deletion of the Pnpla3 gene in mice did not affect TAG hydrolysis, nor did it influence energy/glucose/lipid homoeostasis or hepatic steatosis/injury. Experimental interventions designed to increase Pnpla3 expression (refeeding, high-sucrose diet, diet-induced obesity, and liver X receptor agonism) likewise failed to reveal differences in the above-mentioned metabolic phenotypes. Expression of the Pnpla3 paralog, Pnpla5, was increased in adipose tissue but not in liver of Pnpla3-deficient mice, but compensatory regulation of genes involved in TAG metabolism was not identified. Together these data argue against a role for Pnpla3 loss-of-function in fatty liver disease or metabolic syndrome in mice.

Pubmed ID: 21068004 RIS Download

Mesh terms: Adipose Tissue | Animals | Energy Metabolism | Fatty Liver | Male | Metabolic Syndrome X | Mice | Mice, Knockout | Phospholipases A2, Calcium-Independent | Triglycerides

Research resources used in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Austrian Science Fund FWF, Id: F 3001
  • Agency: NHLBI NIH HHS, Id: P01 HL020948
  • Agency: NHLBI NIH HHS, Id: P01 HL-020948
  • Agency: Austrian Science Fund FWF, Id: Z 136
  • Agency: NHLBI NIH HHS, Id: P01 HL-057278
  • Agency: NHLBI NIH HHS, Id: RL1 HL-092550
  • Agency: NIDDK NIH HHS, Id: P30 DK036836
  • Agency: NHLBI NIH HHS, Id: RL1 HL092550
  • Agency: Howard Hughes Medical Institute, Id: P30-DK-036836
  • Agency: NIDDK NIH HHS, Id: P01 HL057278
  • Agency: NHLBI NIH HHS, Id: F 3002
  • Agency: Austrian Science Fund FWF, Id:

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Joslin Diabetes Center Bioinformatics Core

Core that provides services for diabetes researchers in design, analysis, interpretation, publication, and data release of experiments involving all types of high-throughput omic data_ and their integration with each other, and with clinical and physiological data.

tool

View all literature mentions

Joslin Diabetes Center Flow Cytometry Core

Core that provides cell sorting and flow cytometry services. Specific services include cell analysis, large object sorting,magnetic cell enrichment, and automatic cell counting.

tool

View all literature mentions

Joslin Diabetes Center Advanced Microscopy Core

Core that provides services for performing specific morphological procedures, providing training and access to equipment, maintaining the specialized microscopes, and giving advice and interpretation.

tool

View all literature mentions

Joslin Diabetes Center Animal Physiology Core

Core that provides technically advanced physiological evaluation of metabolism in diabetes, obesity, and their associated complications in rodents for DRC investigators and outside users. It also provides training of investigators and trainees in several physiological procedures.

tool

View all literature mentions

Joslin Diabetes Center Advanced Genomics and Genetics Core

Core that provides services for genetic and genomic analysis, including DNA extraction from blood, access to DNA collections from the Core�s repository, SNP genotyping, and support for gene expression studies based on both high-density oligonucleotide arrays and real-time quantitative PCR.

tool

View all literature mentions

Joslin Diabetes Center Induced Pluripotent Stem Cell Core

Core that maintains a centralized facility for the generation and propagation of reprogrammed iPS cells for use in molecular and cellular pathologies underlying diabetes and its complications.

tool

View all literature mentions

Joslin Diabetes Center Enrichment Core

Six component core which facilitates the exchange of research information and discussions among investigators, fellows and students within the Joslin Diabetes Center, as well as between Joslin Staff and outside researchers with similar interests.

tool

View all literature mentions