Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.

Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the Telomere Interactome, built upon the six core telomeric proteins TRF1, TRF2, RAP1, TIN2, TPP1, and POT1. Of the ∼12,000 human proteins examined, we identified over 300 proteins that associated with the six core telomeric proteins. The majority of the identified proteins have not been previously linked to telomere biology, including regulators of post-translational modifications such as protein kinases and ubiquitin E3 ligases. Results from this study shed light on the molecular niche that is fundamental to telomere regulation in humans, and provide a valuable tool to investigate signaling pathways in mammalian cells.

Pubmed ID: 21044950


  • Lee OH
  • Kim H
  • He Q
  • Baek HJ
  • Yang D
  • Chen LY
  • Liang J
  • Chae HK
  • Safari A
  • Liu D
  • Songyang Z


Molecular & cellular proteomics : MCP

Publication Data

February 4, 2011

Associated Grants

  • Agency: NCI NIH HHS, Id: CA133249
  • Agency: NIGMS NIH HHS, Id: GM081627
  • Agency: NCI NIH HHS, Id: P30CA125123
  • Agency: NICHD NIH HHS, Id: P30HD024064

Mesh Terms

  • Bacterial Proteins
  • Flow Cytometry
  • Genetic Complementation Test
  • Genome
  • Humans
  • Luminescent Proteins
  • Protein Interaction Mapping
  • Proteins
  • Proteome
  • Retroviridae
  • Signal Transduction
  • Telomere
  • Telomere-Binding Proteins
  • Ubiquitin
  • Ubiquitin-Protein Ligases