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Dual RMCE for efficient re-engineering of mouse mutant alleles.

Nature methods | Nov 29, 2010

http://www.ncbi.nlm.nih.gov/pubmed/20953177

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

Pubmed ID: 20953177 RIS Download

Mesh terms: Alleles | Animals | Basic Helix-Loop-Helix Transcription Factors | Carrier Proteins | Embryonic Stem Cells | Genetic Engineering | Mice | Nerve Tissue Proteins | Nuclear Proteins | Promoter Regions, Genetic | Recombinases | Recombination, Genetic | Smad4 Protein

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Associated grants

  • Agency: Wellcome Trust, Id: 077188

Addgene (Reagent, Plasmid)

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