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Dual RMCE for efficient re-engineering of mouse mutant alleles.

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

Pubmed ID: 20953177

Authors

  • Osterwalder M
  • Galli A
  • Rosen B
  • Skarnes WC
  • Zeller R
  • Lopez-Rios J

Journal

Nature methods

Publication Data

November 29, 2010

Associated Grants

  • Agency: Wellcome Trust, Id: 077188

Mesh Terms

  • Alleles
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Carrier Proteins
  • Embryonic Stem Cells
  • Genetic Engineering
  • Mice
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Recombinases
  • Recombination, Genetic
  • Smad4 Protein