Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A genetic defect in exportin-5 traps precursor microRNAs in the nucleus of cancer cells.

Cancer cell | Oct 19, 2010

http://www.ncbi.nlm.nih.gov/pubmed/20951941

The global impairment of mature microRNAs (miRNAs) is emerging as a common feature of human tumors. One interesting scenario is that defects in the nuclear export of precursor miRNAs (pre-miRNAs) might occur in transformed cells. Exportin 5 (XPO5) mediates pre-miRNA nuclear export and herein we demonstrate the presence of XPO5-inactivating mutations in a subset of human tumors with microsatellite instability. The XPO5 genetic defect traps pre-miRNAs in the nucleus, reduces miRNA processing, and diminishes miRNA-target inhibition. The XPO5 mutant form lacks a C-terminal region that contributes to the formation of the pre-miRNA/XPO5/Ran-GTP ternary complex and pre-miRNAs accumulate in the nucleus. Most importantly, the restoration of XPO5 functions reverses the impaired export of pre-miRNAs and has tumor-suppressor features.

Pubmed ID: 20951941 RIS Download

Mesh terms: Cell Line, Tumor | Cell Nucleus | Down-Regulation | Gene Expression Regulation, Neoplastic | Gene Silencing | Humans | Karyopherins | MicroRNAs | Mutant Proteins | Mutation | Neoplasms | Phenotype | RNA Precursors | RNA Processing, Post-Transcriptional | Transfection | Tumor Suppressor Proteins | ran GTP-Binding Protein