Functional redundancy of paralogs of an anaphase promoting complex/cyclosome subunit in Caenorhabditis elegans meiosis.
The anaphase promoting complex/cyclosome (APC/C) mediates the metaphase-to-anaphase transition by instructing the ubiquitination and turnover of key proteins at this stage of the cell cycle. We have recovered a gain-of-function allele in an APC5 subunit of the anaphase promoting complex/cyclosome. This finding led us to investigate further the role of APC5 in Caenorhabditis elegans, which contains two APC5 paralogs. We have shown that these two paralogs, such-1 and gfi-3, are coexpressed in the germline but have nonoverlapping expression patterns in other tissues. Depletion of such-1 or gfi-3 alone does not have a notable effect on the meiotic divisions; however, codepletion of these two factors results in meiotic arrest. In sum, the two C. elegans APC5 paralogs have a redundant function during the meiotic divisions.
Pubmed ID: 20944012
- Stein KK
- Nesmith JE
- Ross BD
- Golden A
December 15, 2010
- Anaphase-Promoting Complex-Cyclosome
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Gene Expression Regulation
- Protein Subunits
- Tissue Distribution
- Ubiquitin-Protein Ligase Complexes