• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

Pubmed ID: 20927106

Authors

  • Sheard LB
  • Tan X
  • Mao H
  • Withers J
  • Ben-Nissan G
  • Hinds TR
  • Kobayashi Y
  • Hsu FF
  • Sharon M
  • Browse J
  • He SY
  • Rizo J
  • Howe GA
  • Zheng N

Journal

Nature

Publication Data

November 18, 2010

Associated Grants

  • Agency: NIDDK NIH HHS, Id: P30 DK056341
  • Agency: NIDDK NIH HHS, Id: P30 DK056341-10
  • Agency: NIAID NIH HHS, Id: R01 AI068718
  • Agency: NIAID NIH HHS, Id: R01 AI068718-04
  • Agency: NCI NIH HHS, Id: R01 CA107134
  • Agency: NCI NIH HHS, Id: R01 CA107134
  • Agency: NCI NIH HHS, Id: R01 CA107134-07
  • Agency: NIGMS NIH HHS, Id: R01 GM057795
  • Agency: NIGMS NIH HHS, Id: R01 GM057795-12
  • Agency: NIAID NIH HHS, Id: R01AI068718
  • Agency: NIGMS NIH HHS, Id: R01GM57795
  • Agency: NIGMS NIH HHS, Id: T32 GM07270
  • Agency: Howard Hughes Medical Institute, Id:
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Amino Acid Sequence
  • Amino Acids
  • Arabidopsis
  • Arabidopsis Proteins
  • Binding Sites
  • Crystallography, X-Ray
  • Cyclopentanes
  • F-Box Proteins
  • Indenes
  • Inositol Phosphates
  • Isoleucine
  • Models, Molecular
  • Molecular Sequence Data
  • Oxylipins
  • Peptide Fragments
  • Plant Growth Regulators
  • Protein Binding
  • Protein Structure, Tertiary
  • Repressor Proteins
  • Signal Transduction