Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Publication

Expression of pax6 and sox2 in adult olfactory epithelium.

The Journal of comparative neurology | 2010

The olfactory epithelium maintains stem and progenitor cells that support the neuroepithelium's life-long capacity to reconstitute after injury. However, the identity of the stem cells--and their regulation--remain poorly defined. The transcription factors Pax6 and Sox2 are characteristic of stem cells in many tissues, including the brain. Therefore, we assessed the expression of Pax6 and Sox2 in normal olfactory epithelium and during epithelial regeneration after methyl bromide lesion or olfactory bulbectomy. Sox2 is found in multiple kinds of cells in normal epithelium, including sustentacular cells, horizontal basal cells, and some globose basal cells. Pax6 is co-expressed with Sox2 in all these, but is also found in duct/gland cells as well as olfactory neurons that innervate necklace glomeruli. Most of the Sox2/Pax6-positive globose basal cells are actively cycling, but some express the cyclin-dependent kinase inhibitor p27(Kip1), and are presumably mitotically quiescent. Among globose basal cells, Sox2 and Pax6 are co-expressed by putatively multipotent progenitors (labeled by neither anti-Mash1 nor anti-Neurog1) and neuron-committed transit amplifying cells (which express Mash1). However, Sox2 and Pax6 are expressed by only a minority of immediate neuronal precursors (Neurog1- and NeuroD1-expressing). The assignment of Sox2 and Pax6 to these categories of globose basal cells is confirmed by a temporal analysis of transcription factor expression during the recovery of the epithelium from methyl bromide-induced injury. Each of the Sox2/Pax6-colabeled cell types is at a remove from the birth of neurons; thus, suppressing their differentiation may be among the roles of Sox2/Pax6 in the olfactory epithelium.

Pubmed ID: 20852734 RIS Download

Research resources used in this publication

None found

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

Agency: NIDCD NIH HHS, United States
Id: R01 DC002167-10
Agency: NIDCD NIH HHS, United States
Id: DC002167
Agency: NIDCD NIH HHS, United States
Id: R01 DC002167
Agency: NIDCD NIH HHS, United States
Id: F30 DC010276-01
Agency: NIDCD NIH HHS, United States
Id: R01 DC002167-13
Agency: NIDCD NIH HHS, United States
Id: R01 DC002167-14
Agency: NIDCD NIH HHS, United States
Id: F30 DC010276
Agency: NIDCD NIH HHS, United States
Id: R01 DC002167-12
Agency: NIDCD NIH HHS, United States
Id: R01 DC002167-11
Agency: NIDCD NIH HHS, United States
Id: F30 DC010276-03
Agency: NIDCD NIH HHS, United States
Id: F30 DC010276-02

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.